منابع مشابه
Relationship Between Oxalate, Oxalate Oxidase Activity, Oxalate Sensitivity, and White Mold Susceptibility in Phaseolus coccineus.
ABSTRACT Sclerotinia sclerotiorum is a necrotrophic pathogen that devastates the yields of numerous crop species, including beans. The disease in common bean and pea is referred to as white mold. We examined the relationship between oxalate, an established virulence factor of S. sclerotiorum, and partial white mold resistance of scarlet runner bean (Phaseolus coccineus). P. coccineus genotypes ...
متن کاملDetermination of urinary oxalate with commercially available oxalate oxidase.
For this direct colorimetry of urinary oxalate, commercially available oxalate oxidase (EC 1.2.3.4) is used. The urine is first diluted, to diminish the effect of interfering substances. Analytical recovery of oxalate from urines with five different oxalate concentrations (0.4 to 2.0 mmol/L) ranged from 92 to 109% (mean 99%). The within-day and between-day precision (CV) of the method for a wid...
متن کاملAssay of plasma oxalate with soluble oxalate oxidase.
We use oxalate oxidase from barley seedlings for the colorimetric determination of oxalate in plasma. The oxalate is converted to hydrogen peroxide, which, in the presence of peroxidase, is detected by a Trinder-like chromogenic system. Optimization of the assay, including deproteinization and elimination of interferences from reducing substrates, is described. Ascorbate additions (200 mumol/L)...
متن کاملStructure and function studies of oxalate oxidase.
Oxalate oxidase (E.C. 1.2.3.4) catalyses the conversion of oxalate and dioxygen to carbon dioxide and hydrogen peroxide (for reviews see [ 1-31). Oxalate oxidase activity has been detected in a number of organisms but the best biochemically characterised enzymes are from barley and wheat. The plant enzyme is isolated as a glycosylated homopentamer which is remarkably resistant to heat, SDS and ...
متن کاملDetermination of oxalate in foodstuffs with arylamine glass-bound oxalate oxidase and peroxidase
CURRENT SCIENCE, VOL. 81, NO. 3, 10 AUGUST 2001 248 by repeating the experiment at least once. A total of 34 decamer primers (series OPA, OPB etc.) from Operon Tech, CA, USA were screened using three representative genomic DNA samples of black pepper. Of these, 24 primers that yielded consistent and clear band patterns were selected for the final analysis of the 22 accessions used in the presen...
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ژورنال
عنوان ژورنال: Journal of the Institute of Brewing
سال: 2009
ISSN: 0046-9750
DOI: 10.1002/j.2050-0416.2009.tb00374.x